Coding
Part:BBa_K3788004:Design
Designed by: Rebecca Pagès Group: iGEM21_Aix-Marseille (2021-09-27)
6His-P20, Flag-Cry11Aa and Strep-Cyt1Aa coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 154
Illegal XhoI site found at 2438 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.
Source
The 6His_P20__Flag_cry11Aa__Strep_cyt1Aa sequence is made from BBa_K3788002, BBa_K3788001 (BBa_K3788003) and BBa_K3788000 parts. Theses 2 parts are optimised sequences for E. coli obtain from Part:BBa_K2938008, Part:BBa_K2938005 and Part:BBa_K2938003.