Coding

Part:BBa_K3788004:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 19:12, 27 September 2021 by Registry (Talk | contribs)

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6His-P20, Flag-Cry11Aa and Strep-Cyt1Aa coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
    Illegal XhoI site found at 2438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.


Source

The 6His_P20__Flag_cry11Aa__Strep_cyt1Aa sequence is made from BBa_K3788002, BBa_K3788001 (BBa_K3788003) and BBa_K3788000 parts. Theses 2 parts are optimised sequences for E. coli obtain from Part:BBa_K2938008, Part:BBa_K2938005 and Part:BBa_K2938003.


References