Coding

Part:BBa_K3788004

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 19:12, 27 September 2021 by Registry (Talk | contribs)

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6His-P20, Flag-Cry11Aa and Strep-Cyt1Aa coding sequence

The Part BBa_K3788004 is a part composed with BBa_K3788003 (6His_P20__Flag_cry11Aa) and BBa_K3788000 (Strep_cyt1Aa). The chaperon P20 allow the well stability of Cry11Aa and Cyt1Aa. Expressed in operon, it allow a better production of Cry11Aa and Cyt1Aa. Theses 3 proteins proteins are Bacillus thuringiensis proteins. P20 chaperon have a histidin tag in Nter, Cry11Aa toxin have a Flag tag in Nter and Cyt1Aa toxin have a Strep tag II in Nter to allow to purified proteins P20, Cyt1Aa and Cry11Aa; and allow the detection and specific identification by WesterBlot. The gene was optimised for E. coli K12. To allow the expression in operon, an RBS sequence is added before the ATG of Flag_cry11Aa gene sequence and Strep_cyt1Aa gene sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
    Illegal XhoI site found at 2438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None