Coding

Part:BBa_K3788003

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 18:46, 27 September 2021 by Registry (Talk | contribs)

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6His-P20 and Flag-Cry11Aa coding sequence

The Part BBa_K3788003 is a part composed with BBa_K3788002 (6his_P20) and BBa_K3788001 (Flag_cry11Aa). The chaperon P20 allow the well stability of Cry11Aa. Expressed in operon, it allow a better production of Cry11Aa. Both proteins are Bacillus thuringiensis proteins. P20 chaperon have a histidin tag in Nter, Cry11Aa toxin have a Flag tag in Nter to allow to purified proteins P20 and Cry11Aa; and allow the detection and specific identification by WesterBlot. The gene was optimised for E. coli K12. To allow the expression in operon, an RBS sequence is added before the ATG of Flag_cry11Aa gene sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
    Illegal XhoI site found at 2438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None