Coding

Part:BBa_K3788002

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 18:20, 27 September 2021 by Registry (Talk | contribs)

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6His-P20

BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis. This chaperon have a histidin tag in Nter to allow to purified the protein P20 and allow the detection and specific identification of P20 by WesterBlot. The gene p20 was optimised for E. coli K12.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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