Coding

Part:BBa_K3788001

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 17:57, 27 September 2021 by Registry (Talk | contribs)

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Flag-Cry11Aa

BBa_K3788001 sequence is coding for Cry11Aa, a toxins from Bacillus thuringiensis (Bti). This toxins have a Flag tag in Nter to allow to purified the protein Cry11Aa and allow the detection and specific identification of Cry11Aa by WesterBlot. The gene cry11Aa was optimised for E. coli K12.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1854
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None