Coding

Part:BBa_K3788000

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 15:41, 27 September 2021 by Registry (Talk | contribs)

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StrepII-Cyt1Aa

BBa_K3788000 sequence is coding for Cyt1Aa, a toxins from Bacillus thuringiensis. This toxins have a strep tag II in Nter to allow to purified the protein Cyt1Aa and allow the detection and specific identification of Cyt1Aa by WesterBlot. The gene cyt1Aa was optimised for E. coli K12. Cyt1Aa can target insects membranes.

strepII_cyt1Aa gene is 777pB Strep_Cyt1Aa protein is 28,5 kDa

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None