Coding

Part:BBa_K3787001

Designed by: Leung Gabriel, Tam Ching Yeung, Wong Chun Hei   Group: iGEM21_HK_GTC   (2021-09-27)
Revision as of 13:51, 27 September 2021 by Registry (Talk | contribs)

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WT PETase-MHETase

This part is a chimeric protein used to depolymerize PET into its constituting monomers. It consists of two enzymes, PETase and MHETase. The PETase part degrades PET into bis(2-hydroxyethyl) terephthalic acid (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), and terephthalic acid (TPA), while the MHETase part degrades MHET into TPA and ethylene glycol (EG). The C-terminus of PETase is linked to the N-terminus of MHETase using a 12 amino acid serine-glycine linker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
    Illegal NheI site found at 1038
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
    Illegal NotI site found at 1586
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
    Illegal NgoMIV site found at 1513
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part
//function/degradation
Parameters
protein