Signalling
Part:BBa_K3804010
Designed by: iGEM21_UTokyo Group: iGEM21_UTokyo (2021-09-26)
Reporter for quorum sensing systems in yeast with LasR
We created this part with reference to BBa_K1024002in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, LasR, ADH1 terminator, pLasR, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator and CYC1terminator and changed LuxR to LasR, pLux to pLas. Las R is homolog of LusR. The LasR gene is constitutively expressed. On the other hands, activation of pLas occurs only when sufficient amount of AHL exist in the environment. When pLas was activated, mCherry is expressed and lets us know the existence of AHL.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2567
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1580
Illegal BamHI site found at 2738 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1928
Illegal AgeI site found at 2125 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 506
Illegal BsaI site found at 893
Illegal BsaI site found at 1280
Illegal BsaI.rc site found at 547
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1321
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Categories
Parameters
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