Coding

Part:BBa_K3804002

Designed by: iGEM21_UTokyo   Group: iGEM21_UTokyo   (2021-09-24)
Revision as of 11:38, 27 September 2021 by ISS1322 (Talk | contribs)


Qsc receptor

This is a Qsc receptor which is designed to use in S.Cerevisiae. QscR bind with AHL and form AHL-QscR complex. Two AHL-QscR complexes form　a dimer and activate pQscR. Originally, pQscR exists in Pseudomonas aeruginosa and is used for quorum sensing. In Pseudomonas aeruginosa, AHL is expressing constitutively and the amount of AHL is related to the density of Pseudomonas aeruginosa. We create this part in order to detect Pseudomonas aeruginosa by using the part in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
    Illegal XhoI site found at 25
    Illegal XhoI site found at 76
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 241


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Categories
Parameters
None