Composite

Part:BBa_K3715071

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-27)
Revision as of 09:22, 27 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


T7 promoter+RBS+ His+Linker f+SUMO+Linker d+PETase_MT13+Linker e+T7 terminator

This part consists of T7 promoter, RBS, protein coding sequence(His+Linker f+Sumo+Linker d+PETase_MT13+ Linker e) and T7 terminator,and the biological module can be build into E.coli for protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 296
    Illegal PstI site found at 1264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 296
    Illegal NheI site found at 73
    Illegal PstI site found at 1264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 296
    Illegal BglII site found at 185
    Illegal BamHI site found at 1285
    Illegal XhoI site found at 384
    Illegal XhoI site found at 1271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 296
    Illegal PstI site found at 1264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 296
    Illegal PstI site found at 1264
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None