Generator

Part:BBa_K3740047

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-09-27)
Revision as of 09:13, 27 September 2021 by Registry (Talk | contribs)

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J23100-B0034-bphS-pET RBS-bphO-rrnB T1

Near-infrared light activated synthesis of c-di-GMP in Acetobacter xylinum

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 189
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 294
    Illegal BglII site found at 1357
    Illegal XhoI site found at 632
    Illegal XhoI site found at 2489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 447
    Illegal NgoMIV site found at 621
    Illegal NgoMIV site found at 938
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 2678
    Illegal AgeI site found at 2634
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 982
    Illegal BsaI.rc site found at 482
    Illegal BsaI.rc site found at 1456


[edit]
Categories
Parameters
None