Coding

Part:BBa_K3789002:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)
Revision as of 03:47, 27 September 2021 by EmmaMei (Talk | contribs)

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PNP1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 163
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PNP1 gene sequence with a common secretion signal sequence “mating factor alpha prepro-leader (MF alpha)” is inserted into The yeast strain FY4. The selection of yeast gene sequence reduces the possibility of rejection of foreign genes.



Source

The sequence we used comes from Saccharomyces cerevisiae S288c


References