Composite

Part:BBa_K3832007:Design

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 15:51, 26 September 2021 by SuperCRISPR (Talk | contribs) (→‎References)

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Toggle-switch circuit with GFP and RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2854
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2854
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2854
    Illegal AgeI site found at 1891
    Illegal AgeI site found at 3436
    Illegal AgeI site found at 3548
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sfGFP and mRFP1 are functioned as reporter here. Target gene should be inserted between CDS of reporter gene and the terminator. The efficiency of RBS followed by repressor genes are crucial to realize the bistable statement of toggle switch circuit. Trying to insert gene or other sequence between promoter and RBS is Not Recommanded.


Source

Sequences are derived from existing parts in iGEM registry. DNA samples are De-novo synthesized.

References

[1]Timothy S. Gardner, Charles R. Cantor, James J. Collins, Construction of a genetic toggle switch in Escherichia coli Supplementary Information, Nature volume 403, pages 339–342 (2000)