Coding

Part:BBa_K3863006

Designed by: LEONG WENG I   Group: iGEM21_PuiChing_Macau   (2021-09-26)
Revision as of 14:37, 26 September 2021 by Registry (Talk | contribs)

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a-amylase-HA-glucoamylase-his

While an amylase ought to be capable of degrading starch, the product protein is believed to be periplasmic and thus ought to only degrade starch if it leaks from the periplasm in significant quantities. Its natural function inE. colipresumably involves degrading shorter glucose chains:Lengsfeldet al(2008)state that "MalS produces preferentially maltohexaose from longer maltodextrins in the periplasm".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1287
    Illegal NotI site found at 2146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2441
    Illegal NgoMIV site found at 2633
    Illegal NgoMIV site found at 2637
    Illegal NgoMIV site found at 3275
    Illegal NgoMIV site found at 3649
    Illegal NgoMIV site found at 3803
    Illegal NgoMIV site found at 3824
    Illegal AgeI site found at 423
    Illegal AgeI site found at 1588
    Illegal AgeI site found at 2393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3026


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Categories
Parameters
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