RBS

Part:BBa_K3832004

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 11:43, 26 September 2021 by SuperCRISPR (Talk | contribs)


medium RBS

This part is a medium RBS in E.coli, which has been characterized by Timothy S. Gardner & James J. Collins[1]. It can be used to construct a bistable toggle switch circuit. The efficiency data can be seen in the Reference (RBS-D in the article) Reference of parts 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1]Timothy S. Gardner, Charles R. Cantor, James J. Collins, Construction of a genetic toggle switch in Escherichia coli Supplementary Information, Nature volume 403, pages 339–342 (2000)

[edit]
Categories
//rbs/prokaryote
Parameters
biologyE.coli
rbsMedium