Composite

Part:BBa_K3832001

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 07:56, 26 September 2021 by Dan012 (Talk | contribs)

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Induced expression circuit of aroG (Mutant s211f)

This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2554
    Illegal NheI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2541
    Illegal XhoI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 453


[edit]
Categories
//cds/enzyme/ligase
Parameters
biologyE.coli
keggEC 2.5.1.54
proteinaroG