Coding

Part:BBa_K3832000

Designed by: Hongyu Chen,Dan Tan   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 05:55, 26 September 2021 by Registry (Talk | contribs)

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aroG (Mutant S211F)

This part encodes a mutant of AroG (3-deoxy-7-phosphoheptulonate synthase, EC 2.5.1.54 ), in which serine at 211 was replaced by phenylalanine. The mutant can relieve the allosteric inhibition of phenylalanine, thus increasing the catalytic rate and downstream product yield.

The enzyme that encoded by the sequence catalyze the following reactions: phosphoenolpyruvate+D-erythrose 4-phosphate+H2O =3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate +phosphate

Derived from E.coli DH5alpha strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/ligase
Parameters
biologyE.coli
keggEC 2.5.1.54
proteinaroG