Coding

Part:BBa_K3866023

Designed by: Pericles Vasileiou   Group: iGEM21_Thessaly   (2021-09-24)
Revision as of 19:49, 25 September 2021 by Periclesv (Talk | contribs)


sbm - Methylmalonyl-CoA mutase GB compatible with B2-B5

Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA. Could be part of a pathway that converts succinate to propionate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1697
    Illegal BamHI site found at 985
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1697
    Illegal NgoMIV site found at 2012
    Illegal AgeI site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Haller T, Buckel T, Rétey J, Gerlt JA (2000). Discovering newenzymes and metabolic pathways: conversion of succinate to propionate by Escherichia coli. Biochemistry, 39:4622–4629. https://doi.org/10.1021/bi992888d

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Categories
Parameters
None