Translational_Unit

Part:BBa_K3866016

Designed by: Ioanna Gkoni   Group: iGEM21_Thessaly   (2021-09-23)
Revision as of 17:20, 25 September 2021 by Igkoni (Talk | contribs)


Plac-MazE-double terminator


Fig.2:J23115 with RBS

Usage and Biology

This part includes the weak promoter of the lactose operon without the lactose operator,which is used for protein expression. Also, it includes the antitoxin MazE that will be utilized to the toxin-antitoxin system and the double terminator.



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha 2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

i

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Categories
Parameters
None