Coding

Part:BBa_K3866008

Designed by: Ioanna Gkoni   Group: iGEM21_Thessaly   (2021-09-23)
Revision as of 16:53, 25 September 2021 by Igkoni (Talk | contribs)


MazE antitoxin GB compatible with B3-B5

MazE antitoxin is used in the toxin-antitoxin system with mazF toxin. This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity.

Fig.2:J23115 with RBS

Usage and Biology

The MazEF system is an extensively studied type II TA module.Toxins in type II TA systems not only inhibit DNA, RNA, or protein synthesis but are also involved in antibiotic resistance, biofilm formation, and persistent cell formation in the plasmid-based systems



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.

Fig.1:The overhangs of this part in the GoldenBraid Grammar


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

===References===

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Categories
Parameters
None