Regulatory

Part:BBa_K3804001

Designed by: Rintaro Shimojo   Group: iGEM21_UTokyo   (2021-09-25)
Revision as of 16:43, 25 September 2021 by Registry (Talk | contribs)

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Yeast Anb1 promoter

There are many genes in yeast that are regulated by oxygens supply. ANB1 is one of the genes controlled by oxygen concentration. Many kinds of oxygen-dependent repressors are bind to the upstream region of the ANB1 ORF and regulate the expression. Especially, ROX1 repressor protein takes a major role in this regulation. This part is about 0.5kbp upstream region of ANB1 ORF and it works as a promoter. In the plasmid inserted environment, the Anb1 promoter functions as a promoter that is repressed under anaerobic conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None