Regulatory

Part:BBa_K3866003

Designed by: Ioanna Gkoni   Group: iGEM21_Thessaly   (2021-09-23)
Revision as of 15:55, 25 September 2021 by Igkoni (Talk | contribs)


Partial T5 Promoter - CuO- RBS GB compatible with A1-B2

Partial T5 promoter is a strong constitutive Promoter that can be used in the toxin-antitoxin system with the Cym repressor that our team uses in their system.

Fig.2:J23115 with RBS

Usage and Biology

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position A1-B2.

Fig.1:The overhangs of this part in the GoldenBraid Grammar


Experimental Use and Experience

This part is used in BBa_K3505033 BBa_K3505035

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

===References===

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Categories
Parameters
None