Coding
Part:BBa_K3715105
Designed by: Yuan Wang Group: iGEM21_TJUSLS_China (2021-09-25)
T7 promoter+RBS+Linker a+His+Super1+Linker b+T7 terminator
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super is a complicated mutation of PETase, which contains 11 mutation sites:S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-I208F.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 863
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 863
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 878
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 863
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 863
Illegal NgoMIV site found at 138
Illegal NgoMIV site found at 192
Illegal NgoMIV site found at 219 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |