Coding

Part:BBa_K3866002

Designed by: Venetios Michelioudakis   Group: iGEM21_Thessaly   (2021-09-23)
Revision as of 13:44, 25 September 2021 by Venetios (Talk | contribs) (→‎Usage and Biology)



ackA-Acetate kinase GB compatible with B3-B5


Usage and Biology

AckA catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. It is utilized from our system for the reverse reaction in order to produce Acetate.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.


Figure 1.The overhangs of this part in the GoldenBraid Grammar.

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction Digestion of LacI with EcoRI + SlaI, Expected bands : 3189 +2300 + 892 bp, Positive Result:C2

Source

Synthesized by Twist Biosciences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
9/6/2021

[edit]
Categories
Parameters
None