Signalling

Part:BBa_K3804009:Design

Designed by: iGEM21_UTokyo   Group: iGEM21_UTokyo   (2021-09-25)
Revision as of 12:53, 25 September 2021 by Registry (Talk | contribs)

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Reporter for quorum sensing systems in yeast with qscR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1673
    Illegal XhoI site found at 1595
    Illegal XhoI site found at 1646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1811


Design Notes

No special design consideration.


Source

pTEF tVP16 NLS LasR ADH1 terminator pQscR cyc100mini mCherry CYC1 terminator

References