Coding

Part:BBa_K3866002

Designed by: Venetios Michelioudakis   Group: iGEM21_Thessaly   (2021-09-23)
Revision as of 11:42, 25 September 2021 by Venetios (Talk | contribs)



ackA-Acetate kinase GB compatible with B3-B5


Usage and Biology

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.


Figure 1.The overhangs of this part in the GoldenBraid Grammar.

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction Digestion of LacI with EcoRI + SlaI, Expected bands : 3189 +2300 + 892 bp, Positive Result:C2

Source

Synthesized by Twist Biosciences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
9/6/2021

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Categories
Parameters
None