Coding

Part:BBa_K3715001:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-25)
Revision as of 06:20, 25 September 2021 by YuanWang (Talk | contribs) (→‎References)


Super1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 783
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 783
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 783
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 783
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 112
    Illegal NgoMIV site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We know this patr from a novel（https://dx.doi.org/10.1021/acscatal.0c05126）. DuraPETase is another wild type as well as PETase.


Source

Recently, a novel devised a computational strategy (greedy accumulated strategy for protein engineering, GRAPE) to improve the robustness of a PETase fromIdeonella sakaiensis.

References

https://dx.doi.org/10.1021/acscatal.0c05126