Composite

Part:BBa_K3796206

Designed by: Yang Feiyue   Group: iGEM21_CAU_China   (2021-09-24)
Revision as of 09:47, 24 September 2021 by Registry (Talk | contribs)

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PgsiB-rbs-gfp-terminator

This part is composed of an inducible promoter PgsiB, a rbs sequence in Corynebacterium glutamicum (BBa_K2963006), the fluorescent protein gene bjGFP, and the rrnB T1 and T2 terminators (BBa_K3568009). P-gsiB is a sigma factor B dependent promoter from Bacillus subtilis and can be activated under various mild stress, including salt, alkaline, heat shock, ethanol and glucose limitation. Under these stresses, the gfp will have higher expression, leading to stronger fluorescence intensity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None