Composite
Part:BBa_K3796206
Designed by: Yang Feiyue Group: iGEM21_CAU_China (2021-09-24)
PgsiB-rbs-gfp-terminator
This part is composed of an inducible promoter PgsiB, a rbs sequence in Corynebacterium glutamicum (BBa_K2963006), the fluorescent protein gene bjGFP, and the rrnB T1 and T2 terminators (BBa_K3568009). P-gsiB is a sigma factor B dependent promoter from Bacillus subtilis and can be activated under various mild stress, including salt, alkaline, heat shock, ethanol and glucose limitation. Under these stresses, the gfp will have higher expression, leading to stronger fluorescence intensity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |