Coding

Part:BBa_K3796205:Design

Designed by: Yang Feiyue   Group: iGEM21_CAU_China   (2021-09-24)
Revision as of 09:16, 24 September 2021 by Yang Feiyue (Talk | contribs) (→‎Source)


bjGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Users may do furthur codon-optimiziation before using this part. According to literature, this variant is particularly suitable for use in B.diazoefficiens.


Source

This part is originally chemical syhthesized and is usually carried by the plasmid pRJPaph-bjGFP.

References

[1] Ledermann R , Ba Rtsch I , Remus-Emsermann M N , et al. Stable Fluorescent and Enzymatic Tagging of Bradyrhizobium diazoefficiens to Analyze Host-Plant Infection and Colonization[J]. Molecular plant-microbe interactions : MPMI, 2015, 28(9).