Regulatory

Part:BBa_K3796204:Design

Designed by: Yang Feiyue   Group: iGEM21_CAU_China   (2021-09-24)
Revision as of 08:35, 24 September 2021 by Yang Feiyue (Talk | contribs) (→‎References)

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P-atp2 (alkali inducible promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

P-atp2 is a promoter in F0F1 ATPase operon and there is another promoter P-atp1 right in front of it. These two promoters has overlap regions.

Though the minimal promoter region hasn't be verified, the -35 region, -10 region and transcription start site(tss) has been clarified in the literature. Therefore, we exploratively narrow the promoter sequence from 733bp down to 85bp to include merely the essential -35 region, -10 region and tss. Results show that this part can be induced by alkaline effectively as normal.

The full sequence of Patp1-Patp2 has been previously documented by IIT_Delhi(BBa_K1170003).


Source

Corynebacterium glutamicum ATCC 13032

References

[1]Monica, Barriuso-Iglesias, Carlos, et al. Transcriptional analysis of the F0F1 ATPase operon of Corynebacterium glutamicum ATCC 13032 reveals strong induction by alkaline pH[J]. Microbiology, 2006, 152(1):11-21.

[2] Li C , Gao X , Peng X , et al. Intelligent microbial cell factory with genetic pH shooting (GPS) for cell self-responsive base/ acid regulation[J]. Microbial Cell Factories, 2020, 19(1).

[3] Barriuso-Iglesias M , Barreiro C , Sola-Landa A , et al. Transcriptional control of the F 0 F 1 -ATP synthase operon of Corynebacterium glutamicum : SigmaH factor binds to its promoter and regulates its expression at different pH values[J]. Microbial Biotechnology, 2013, 6(2).