Coding
mazF, ydcE

Part:BBa_K3796203:Design

Designed by: Yang Feiyue   Group: iGEM21_CAU_China   (2021-09-23)
Revision as of 07:57, 24 September 2021 by Yang Feiyue (Talk | contribs) (→‎Design Notes)

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ndoA (endoribonuclease toxin in Bacillus subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial start codon in ndoA is "TTG". You may consider changing or adding other start codons to get possible higher expression. For example, XJTU-China added another "ATG" before the initial sequence of ndoA in part: BBa_K3331005.

If you aim to test whether ndoA functions as normal in your chassis, be sure to use an inducible promoter that has little basal level expression, otherwise the bacteria may be all killed before adding any inducer.

Source

Bacillus subtilis subsp. subtilis str. 168

References

[1] Park J H , Yamaguchi Y , Inouye M . Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase[J]. Febs Letters, 2011, 585(15).

[2]Simanshu, DK, Yamaguchi, et al. Structural Basis of mRNA Recognition and Cleavage by Toxin MazF and Its Regulation by Antitoxin MazE in Bacillus subtilis[J]. MOL CELL, 2013, 2013,52(3)(-):447-458.

[3] Pellegrini O , Mathy N , Gogos A , et al. The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor[J]. Molecular Microbiology, 2005, 56(5).

[4] Ishida Y , Park J H , Mao L , et al. Replacement of All Arginine Residues with Canavanine in MazF-bs mRNA Interferase Changes Its Specificity[J]. The Journal of Biological Chemistry, 2013, 288(11).