RNA

Part:BBa_K3885007:Design

Designed by: XIA YAO   Group: iGEM21_ZJUT-China   (2021-09-21)
Revision as of 00:58, 22 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Interacting Guide RNA（igRNA）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project, we add a special sequence in the 5' end of the gRNA, which consists of recognition sequence A, cis-repressed sequence and recognition sequence B. The cis-repressed sequence is complementary to the spacer, leading to the repression of the binding ability between igRNA with its DNA target. The recognition sequence A and the recognition sequence B are complementary to the interacting sequence A and interacting sequence, respectively.


Source

none

References