Terminator

Part:BBa_K3885004:Design

Designed by: XIA YAO   Group: iGEM21_ZJUT-China   (2021-09-21)
Revision as of 00:50, 22 September 2021 by Kkarla (Talk | contribs) (→‎References)

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T500


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T500 is a strong strong intrinsic terminator. We used it as a transcription terminator in our experiments.


Source

A mutant construct derived from the late gene terminator sequence of bacteriophage 82； (Yarnell and Roberts, 1999)

References

Larson, M. H., Greenleaf, W. J., Landick, R., and Block, S. M. (2008) Applied force reveals mechanistic and energetic details of transcription termination, Cell 132, 971­982.