Coding

Part:BBa_K3848000

Designed by: Ashley Suraj Hermon   Group: iGEM21_IISER_Bhopal   (2021-09-21)
Revision as of 19:27, 21 September 2021 by Ash007 (Talk | contribs)


TRAIL-Smac Fusion Protein with HlyA signal peptide

Fusion protein which can specifically induce apoptosis and kills Cancer cells. This fusion protein has N terminal 7 amino acid residues of Smac/DIABLO (small-mitochondria associated activator of Caspase) and the soluble portion of TRAIL (TNF related Apoptosis Inducing Ligand). The smac-n7 is given a Cell Penetrating Peptide (CPP) consisting of 8 Arginine residues for efficient membrane penetration into mammalian cells .A protease cleavage site recognized by Matrix Metalloproteinases (MMP-3 and MMP-9) and Urokinase (uPa) will cleave the Smac portion once the FP reaches cancer cells .It is also linked with HlyA signal peptide (C terminal signal peptide) that allows secretion by Escherichia coli . A 6X-His Tag is given for efficient purification of the protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 655
    Illegal BamHI site found at 661
    Illegal XhoI site found at 197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 775
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 796


[edit]
Categories
Parameters
None