Coding

Part:BBa_K3900000

Designed by: Lennart Lutz   Group: iGEM21_Bielefeld-CeBiTec   (2021-09-21)
Revision as of 15:38, 21 September 2021 by Registry (Talk | contribs)

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Ribose Binding Protein NNK Sequence for Darwin-Assembly

This part is a modified version of the Ribose Binding Protein (RBP). In this modification of the sequence certain triplets are marked as NNK-triplets, resembling a created gene library of RBP-sequences. The sequences were mutated via Darwin-Assembly and the library quality analyzed by Nanopore sequencing. X different sequences were able to be differentiated.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 385
    Illegal PstI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 385
    Illegal PstI site found at 601
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 385
    Illegal PstI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 385
    Illegal PstI site found at 601
    Illegal NgoMIV site found at 480
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None