Coding

Part:BBa_K3972002

Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen   Group: iGEM21_TU-Eindhoven   (2021-09-20)
Revision as of 11:28, 21 September 2021 by Ingrid Kolen (Talk | contribs)


ARG1 (Acoustic Reporter Gene 1)

E. coli codon-optimized ARG1 is a collection of 11 proteins that form gas vesicles when expressed. The proteins involved are GvpA, GvpC, GvpR, GvpN, GvpF, GvpG, GvpL, GvpS, GvpK, GvpJ, GvpT and GvpU.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 851
    Illegal EcoRI site found at 4004
    Illegal XbaI site found at 602
    Illegal PstI site found at 3861
    Illegal PstI site found at 3966
    Illegal PstI site found at 4344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 851
    Illegal EcoRI site found at 4004
    Illegal NheI site found at 736
    Illegal PstI site found at 3861
    Illegal PstI site found at 3966
    Illegal PstI site found at 4344
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 851
    Illegal EcoRI site found at 4004
    Illegal BglII site found at 1355
    Illegal BamHI site found at 4760
    Illegal XhoI site found at 4448
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 851
    Illegal EcoRI site found at 4004
    Illegal XbaI site found at 602
    Illegal PstI site found at 3861
    Illegal PstI site found at 3966
    Illegal PstI site found at 4344
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 851
    Illegal EcoRI site found at 4004
    Illegal XbaI site found at 602
    Illegal PstI site found at 3861
    Illegal PstI site found at 3966
    Illegal PstI site found at 4344
    Illegal NgoMIV site found at 1806
    Illegal NgoMIV site found at 2208
    Illegal AgeI site found at 4727
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
Parameters
biologyAnabaena flos-aqua and Bacillus megaterium
proteinARG1