Part:BBa_K4040015
CAR-MEGF10
Background and structure of CAR-MEGF10
The function of CAR-MEGF10 is the same as the CARγ, which increases the phagocytosis of the macrophages. And the structure is also highly similar to the CARγ, with the only difference lying in the intracellular domain of the CAR, in which the common γ subunit of Fc receptors is replaced by MEGF10.
The mechanism of CAR-MEGF10
After the extracellular αS-scFv domain binds to the S protein of SARS-CoV-2, the intracellular cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) are activated with the help of the Src family kinases. And the SH2 binding domains for the protein expressing SH2 matching domains will be further exposed due to the space conformation change. The protein expressing SH2 matching domains, protein Syk, a phagocytic signaling effector can then active the downstream signaling transduction cascades and initiate the phagocytosis in our CARγ-macrophages eventually.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 344
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 952
Illegal NgoMIV site found at 1288
Illegal NgoMIV site found at 1825
Illegal NgoMIV site found at 1891
Illegal NgoMIV site found at 2050
Illegal AgeI site found at 440 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1168
Illegal BsaI.rc site found at 1432
Illegal SapI.rc site found at 1989
Illegal SapI.rc site found at 2619
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