RNA

Part:BBa_K3904404

Designed by: Bernadeta Aleksandravičiūtė   Group: iGEM21_Vilnius-Lithuania   (2021-09-20)
Revision as of 19:48, 20 September 2021 by Registry (Talk | contribs)

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tyrP-specific sgRNA

Vilnius-Lithuania iGEM 2021 project AmeByelooks at amebiasis holistically and comprehensively, therefor target E. histolytica from several angles: prevention and diagnostics. As a tool to prevent amebiasis, the team created probiotics capable of naringenin biosynthesis. For the diagnostic part, the project includes a rapid, point of care, user-friendly diagnostic test identifying extraintestinal amebiasis. The main components of this test are aptamers, specific to the E. histolytica secreted proteins. These single-stranded DNA sequences fold into tertiary structures for particular fit with target proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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