Coding
Part:BBa_K3853003
Designed by: Gongyu Liu Group: iGEM21_CPU_CHINA (2021-09-20)
dCas9
A Cas9 protein with H840A and D10A mutations has only nucleic acid binding activity, but lacks nucleic acid cleavage activity
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3960
Illegal AgeI site found at 3304 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |