Part:BBa_K3972000

TtrS

E. coli-codon-optimized TtrS(BBa_K3972000) and TtrR (BBa_K3972001) are two basic parts which are derived from the two-component system of the marine bacterium Shewanella baltica. TtrS is the membrane-bound sensor kinase (SK) which can sense tetrathionate outside the cell, and TtrR is the DNA-binding response regulator (RR).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1561
  Illegal PstI site found at 1013
  Illegal PstI site found at 1028
  Illegal PstI site found at 1084
  Illegal PstI site found at 1381
  Illegal PstI site found at 1408
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1561
  Illegal NheI site found at 1309
  Illegal PstI site found at 1013
  Illegal PstI site found at 1028
  Illegal PstI site found at 1084
  Illegal PstI site found at 1381
  Illegal PstI site found at 1408
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1561
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1561
  Illegal PstI site found at 1013
  Illegal PstI site found at 1028
  Illegal PstI site found at 1084
  Illegal PstI site found at 1381
  Illegal PstI site found at 1408
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1561
  Illegal PstI site found at 1013
  Illegal PstI site found at 1028
  Illegal PstI site found at 1084
  Illegal PstI site found at 1381
  Illegal PstI site found at 1408
  Illegal AgeI site found at 783
  
• 1000
  COMPATIBLE WITH RFC[1000]