Project

Part:BBa_K3972003

Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen   Group: iGEM21_TU-Eindhoven   (2021-09-20)
Revision as of 13:13, 20 September 2021 by Registry (Talk | contribs)

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Tetrathionate sensor with activated pTtrB185-269-ARG1

This composite part is a combination of the part BBa_K3972002. The ARG1 proteins are activated by the PttrB185-269 promotor. This promotor is activated by TtrR in the presence of TtrS and tetrathionate. Therefore, in the presence of tetrathionate, TtrS is activated and on its turn activates TtrR. TtrR binds to the PttrB185-269 promotor and induces expression of the ARG1 proteins

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 942
    Illegal EcoRI site found at 4095
    Illegal XbaI site found at 693
    Illegal PstI site found at 3952
    Illegal PstI site found at 4057
    Illegal PstI site found at 4435
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 942
    Illegal EcoRI site found at 4095
    Illegal NheI site found at 827
    Illegal PstI site found at 3952
    Illegal PstI site found at 4057
    Illegal PstI site found at 4435
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 942
    Illegal EcoRI site found at 4095
    Illegal BglII site found at 1446
    Illegal BamHI site found at 4851
    Illegal XhoI site found at 4539
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 942
    Illegal EcoRI site found at 4095
    Illegal XbaI site found at 693
    Illegal PstI site found at 3952
    Illegal PstI site found at 4057
    Illegal PstI site found at 4435
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 942
    Illegal EcoRI site found at 4095
    Illegal XbaI site found at 693
    Illegal PstI site found at 3952
    Illegal PstI site found at 4057
    Illegal PstI site found at 4435
    Illegal NgoMIV site found at 1897
    Illegal NgoMIV site found at 2299
    Illegal AgeI site found at 4818
  • 1000
    COMPATIBLE WITH RFC[1000]


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