Coding

Part:BBa_K3853013

Designed by: RuYi Ma   Group: iGEM21_CPU_CHINA   (2021-09-20)
Revision as of 05:50, 20 September 2021 by Registry (Talk | contribs)

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MnP (E74P)

Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(E74P) is the most promising mutant of MnP, possessing the lowest energy after mutation and would probably has the best stability, but finally showed a sharp decline in enzyme activity, even close to zero.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None