Coding

Part:BBa_K3753000:Design

Designed by: Jiaying Hu   Group: iGEM21_NJTech_China   (2021-09-18)
Revision as of 12:08, 18 September 2021 by Registry (Talk | contribs)

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PAAS-Petunia


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 60
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It should be synthesized with an optimized codon usage for different species.


Source

The coding region of petunia PAAS was amplified by PCR using forward and reverse primers (5'-ATTCATATGGATACTATCAAAATCAACCC-3' and 5'-GGGATCCTTCTACGCATTCAGCATCATAG-3', respectively). (Gen-Bank accession number DQ243784.1)


References