Promoters/Catalog/B. subtilis/Positive

All the promoters on this page are B. subtilis promoters that are positively regulated meaning that increased levels of at least one transcription factor (other than the sigma factor) will increase the activity of these promoters.

Positively regulated B. subtilis σ^A promoters

This section lists promoters that are recognized by E. coli σ⁷⁰ RNAP. σ⁷⁰ is the major E. coli sigma factor so there should be RNAP present to transcribe these promoters under most growth conditions (although maximally during exponential growth).

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Positively regulated B. subtilis σ^B promoters

This section lists promoters that are recognized by E. coli σ^S RNAP. σ^S is the major stationary phase E. coli sigma factor. Use these promoters when you want high promoter activity during stationary phase or during starvation. Since σ^S promoters have the same consensus promoter sequence as σ⁷⁰ promoters, you may find these promoters will be weakly expressed by σ⁷⁰ RNAP.

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