Reporter

Part:BBa_K3829002

Designed by: Yiwen Wang   Group: iGEM21_IvyMaker-China   (2021-09-15)
Revision as of 03:19, 16 September 2021 by Registry (Talk | contribs)

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reporter yeGFP

717bp reporter Enhanced green fluorescent protein produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag.71 We optimized the codon sequence of yeGFP to characterize the strength of promoters The amino acid sequences are presented below: 1 MSKGEELFTG VVPILVELDG 21 DVNGHKFSVS GEGEGDATYG 41 KLTLKFICTT GKLPVPWPTL 61 VTTFGYGVQC FARYPDHMKQ 81 HDFFKSAMPE GYVQERTIFF 101 KDDGNYKTRA EVKFEGDTLV 121 NRIELKGIDF KEDGNILGHK 141 LEYNYNSHNV YIMADKQKNG 161 IKVNFKIRHN IEDGSVQLAD 181 HYQQNTPIGD GPVLLPDNHY 201 LSTQSALSKD PNEKRDHMVL 221 LEFVTAAGIT HGMDELYK


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None