Part:BBa_K3731002

vgb

The vgb can produce HGB so that we can increase bacteria’s capacity of carrying oxygen, which is beneficial to the growth of bacteria.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

iGEM2021_Nanjing-China Experiment

Group: Nanjing-China 2021
Author: Hao Yin

Bacterial hemoglobin (VtHb) is produced by the gram-negative bacterium, Vitreoscilla , in large quantity in response to hypoxic environmental conditions. The vgb gene coding for VtHb has been cloned in E. coli where it is expressed strongly by its natural promoter. The expression of the vgb gene in Vitreoscilla is transcriptionally regulated by oxygen. Vitreoscillu hemoglobin (VHb) is expressed at elevated levels in Vitreoscillu under oxygen-limited cultivation conditions.

Figure1 Structure of mazE

The mazE protein is such a useful antitoxin that it can help bacteria grow longer and survive harsher environment, thus producing more polyP. Thus, we insert mazE gene into E. coli genome to increase the productivity.

Reference:

Zorzini, Buts et al. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding, Nucleic Acids Research, Volume 43, Issue 2, 30 January 2015, Pages 1241–1256