Coding
Part:BBa_K3731001:Design
Designed by: Haoru Song Group: iGEM21_Nanjing-China (2021-09-02)
mazE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR-amplified CFppk1-vgb-mazE was digested with restriction enzymes Kpn I and Hind III, after which it was insert into corresponding multiple cloning sties of the broad-host-range expressing vector pBBR1MCS2.
Source
E.Coli BL21 was purchased from China Center of Industrial Culture Collection (CICC, China). For the construction of pBBR1MCS2-ppk1-vgb-mazE, genomic DNA of E.Coli BL21 was used as the template to PCR-amplify ppk1, vgb and mazE with primers.
References
Zorzini, Buts et al. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding, Nucleic Acids Research, Volume 43, Issue 2, 30 January 2015, Pages 1241–1256