Regulatory
PaccD

Part:BBa_K3758006

Designed by: Michael Burgis   Group: iGEM21_Marburg   (2021-09-12)
Revision as of 14:28, 13 September 2021 by CedKB (Talk | contribs)


PaccD, accD promoter (-82 to +23)

accD promoter, -82 to +23 slight increase from native when transgene attached. Decreased transgene expression if another 5'UTR longer than 129 bp is used in between transgene


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Hirata, N., Yonekura, D., Yanagisawa, S., & Iba, K. (2004). Possible Involvement of the 5′-Flanking Region and the 5′UTR of Plastid accD Gene in NEP-Dependent Transcription. Plant and Cell Physiology, 45(2), 176–186. https://doi.org/10.1093/pcp/pch021

Occhialini, A., Piatek, A. A., Pfotenhauer, A. C., Frazier, T. P., Stewart, C. N., Jr., & Lenaghan, S. C. (2019). MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology. Plant Physiology, 179(3), 943–957. https://doi.org/10.1104/pp.18.01220

[edit]
Categories
//chassis/eukaryote/ntabacum
//chassis/organelle/chloroplast
//direction/forward
//promoter
Parameters
chassisN. tabacum chloroplast