Regulatory
PpsbA core

Part:BBa_K3758003:Design

Designed by: Michael Burgis   Group: iGEM21_Marburg   (2021-09-12)
Revision as of 09:51, 13 September 2021 by CedKB (Talk | contribs) (→‎References)


PpsbA, psbA promoter (-42 to +9) (N. tabacum)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Placeholder Design


Source

Placeholder Source

References

Hayashi, K., Shiina, T., Ishii, N., Iwai, K., Ishizaki, Y., Morikawa, K., & Toyoshima, Y. (2003). A Role of the –35 Element in the Initiation of Transcription at psbA Promoter in Tobacco Plastids. Plant and Cell Physiology, 44(3), 334–341. https://doi.org/10.1093/pcp/pcg041

Occhialini, A., Piatek, A. A., Pfotenhauer, A. C., Frazier, T. P., Stewart, C. N., Jr., & Lenaghan, S. C. (2019). MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology. Plant Physiology, 179(3), 943–957. https://doi.org/10.1104/pp.18.01220