Coding

Part:BBa_K136046

Designed by: alexandra bouaziz   Group: iGEM08_Paris   (2008-10-25)
Revision as of 04:41, 8 November 2008 by Landrain (Talk | contribs)

envZ*

EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR. The resulting phenotype of the mutated strain is complete repression of FlhDC expression and then absence of motility for the bacteria.

EnvZmut.png

This part was successfully used in the composite part BBa_K136066

A site EcoRI is present in the part which doesn't comply with the Biobrick standard. We will soon provide a new version of this part without the EcoRI site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/phosphorylation
//cds/membrane
Parameters
None